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primary antibody mouse igg2a anti human cxcr2 il 8 rb antibody  (R&D Systems)


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    R&D Systems primary antibody mouse igg2a anti human cxcr2 il 8 rb antibody
    Primary Antibody Mouse Igg2a Anti Human Cxcr2 Il 8 Rb Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primary antibody mouse igg2a anti human cxcr2 il 8 rb antibody  (R&D Systems)
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    anti mouse cxcr2 purified rat monoclonal igg2a antibody  (R&D Systems)
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    DEK‐induced migration of LSK and Ly6G + cells is dependent on <t>CXCR2</t> and Gαi protein‐coupled signaling. (A and B) BM Lin – or Ly6G + cells were treated with anti‐rat <t>IgG</t> (isotype), anti‐CXCR2, or anti‐CXCR4 neutralizing antibody prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (A) or Ly6G + (B) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (C and D) BM Lin – or Ly6G + cells were treated with 1000 ng/ml Pertussis toxin (PT) for 4 h at 37°C prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (C) or Ly6G + (D) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (A–D) Data are the mean ± sd of triplicate wells. Data are representative of 1 of 3 separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with control for the given chemokine/recombinant protein
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    anti-human cxcr2/il-8 rb (igg2a; cat. fab331f)  (R&D Systems)
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    DEK‐induced migration of LSK and Ly6G + cells is dependent on <t>CXCR2</t> and Gαi protein‐coupled signaling. (A and B) BM Lin – or Ly6G + cells were treated with anti‐rat <t>IgG</t> (isotype), anti‐CXCR2, or anti‐CXCR4 neutralizing antibody prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (A) or Ly6G + (B) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (C and D) BM Lin – or Ly6G + cells were treated with 1000 ng/ml Pertussis toxin (PT) for 4 h at 37°C prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (C) or Ly6G + (D) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (A–D) Data are the mean ± sd of triplicate wells. Data are representative of 1 of 3 separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with control for the given chemokine/recombinant protein
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    rat anti mouse cxcr2 apc antibody  (R&D Systems)
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    DEK‐induced migration of LSK and Ly6G + cells is dependent on <t>CXCR2</t> and Gαi protein‐coupled signaling. (A and B) BM Lin – or Ly6G + cells were treated with anti‐rat <t>IgG</t> (isotype), anti‐CXCR2, or anti‐CXCR4 neutralizing antibody prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (A) or Ly6G + (B) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (C and D) BM Lin – or Ly6G + cells were treated with 1000 ng/ml Pertussis toxin (PT) for 4 h at 37°C prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (C) or Ly6G + (D) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (A–D) Data are the mean ± sd of triplicate wells. Data are representative of 1 of 3 separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with control for the given chemokine/recombinant protein
    Rat Anti Mouse Cxcr2 Apc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DEK‐induced migration of LSK and Ly6G + cells is dependent on CXCR2 and Gαi protein‐coupled signaling. (A and B) BM Lin – or Ly6G + cells were treated with anti‐rat IgG (isotype), anti‐CXCR2, or anti‐CXCR4 neutralizing antibody prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (A) or Ly6G + (B) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (C and D) BM Lin – or Ly6G + cells were treated with 1000 ng/ml Pertussis toxin (PT) for 4 h at 37°C prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (C) or Ly6G + (D) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (A–D) Data are the mean ± sd of triplicate wells. Data are representative of 1 of 3 separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with control for the given chemokine/recombinant protein

    Journal: Journal of Leukocyte Biology

    Article Title: DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Gαi signaling

    doi: 10.1002/JLB.3AB1120-740R

    Figure Lengend Snippet: DEK‐induced migration of LSK and Ly6G + cells is dependent on CXCR2 and Gαi protein‐coupled signaling. (A and B) BM Lin – or Ly6G + cells were treated with anti‐rat IgG (isotype), anti‐CXCR2, or anti‐CXCR4 neutralizing antibody prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (A) or Ly6G + (B) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (C and D) BM Lin – or Ly6G + cells were treated with 1000 ng/ml Pertussis toxin (PT) for 4 h at 37°C prior to being placed in the top chamber of a transwell plate and allowed to migrate toward 100 ng/ml rhSDF1α, rmDEK, rhIL‐8, or rmMIP2 for 4 h at 37°C. Total LSK (C) or Ly6G + (D) cell migration was determined using flow cytometry with background migration subtracted from total migrated cells. (A–D) Data are the mean ± sd of triplicate wells. Data are representative of 1 of 3 separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared with control for the given chemokine/recombinant protein

    Article Snippet: To block CXCR2 and CXCR4 on the cell surface, BM Lin – and Ly6G + cells were incubated with 2.5 μg/10 6 cells of anti‐mouse CXCR2 purified rat monoclonal IgG2A antibody (R&D Systems; clone 242216), anti‐mouse CXCR4 purified rat monoclonal IgG2B antibody (R&D Systems; clone 247506), or isotype rat IgG control (azide free; R&D Systems; catalog 6‐001‐F) for 30 min at room temperature prior to use and cells washed.

    Techniques: Migration, Flow Cytometry, Control, Recombinant